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Auto Complete: when mini_auto_complete is enabled, a normal AC window will no longer appear.Auto Complete: added the auto_complete_when_likely setting.API: Syntax.name is now derived from the path if not present.API: added missing lect_sheets() method to Python 3.3 plugin host.API: fixed ViewEventListener.on_load_async() sometimes not being called.API: added sublime.CLEAR_TO_RIGHT for use with Window.open_file().Linux: recursively expanding and collapsing sidebar folders now works with alt or super.Code Folding: fixed some edge-case incorrect behaviors.Sidebar: fixed keyboard navigation when the Open Files section is displayed.Auto Complete: fixed a regression with the Definition link.Goto Anything: correct flickering of first tab when filtering files.Goto Anything: add missing ctrl+enter key binding for Windows and Linux.Goto Anything: pasting a file name and line number no longer changes the line in the previous file.Tab Multi-Select: in Goto File, Goto Symbol in Project and Definitions popup, the shift modifier appends the file to the far right.Tab Multi-Select: in Goto File and Goto Symbol in Project, the ctrl/ cmd modifier now opens the file to the right, replacing any existing tabs.Switching files correctly updates the window title and status bar.Mac releases now include native Apple Silicon binaries.Folders in the sidebar can be recursively expanded via alt+arrow key.Fixed a sidebar selection bug with the last file in a folder.Mac: Fixed window title appearing in the wrong location in the Adaptive theme. API: Add missing lected_sheets() method for Python 3.3.Windows: Fix multiple application instances launching using "Open with Sublime Text".Windows: Fix scroll jumping when using auto_hide_menu with adaptive theme.Mac: Improved behavior of Open and Save dialogs.Linux: Use native dialogs functionality in GTK, enabling KDE dialogs when desired.Auto Complete: Fixed an infinite loop when the same folder is added to the sidebar twice.open_files_in_new_window is now supported on all platforms.Add Report a Bug entry in the Help menu.This allows binding a key to run multiple commands without having to use a macro Added the chain command, which accepts a list of commands to run in its "commands" argument.Added Scroll Bar.sublime-mousemap to customize scrollbar interaction.Further performance improvements for Goto Anything.Windows: Fixed an OpenGL regression in 4103.Fix regressions with Goto Anything in 4103.Windows: Fixed an auto complete crash when auto_hide_menu is enabled.Linux, Mac: Caches have been moved to their proper location ( ~/.cache and ~/Library/Caches respectively).Sublime Text 4 (Stable 4107) has been released. Sublime Text/Merge Official Discord chat server:.This gist will no longer be updated as the changelog will be on the official website. Here, we examined the potential source of such chimeric reads and found that they are more likely to be a methodological product, than the result of genuine reverse transcription, integration, and expression. These findings were partly based on the identification of chimeric reads between viral and human RNA in next-generation RNA-sequencing (RNA-seq) data ( Zhang et al., 2020 Ying et al., 2021). Recent studies reported a high frequency of reverse transcription and integration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in infected cells ( Zhang et al., 2020 Ying et al., 2021), with implications for diagnostic detection of SARS-CoV-2 nucleic acids by RT-qPCR and for viral antigen persistence. Indeed, the human genome contains DNA copies of distinct RNA and DNA viruses ( Blinov et al., 2017), as well as numerous retrogenes and pseudogenes ( Baertsch et al., 2008 Richardson et al., 2014 Staszak and Makałowska, 2021), highlighting the possible, albeit infrequent, reverse transcription and integration of non-retroviral RNA into the host genome. The reverse transcriptase and endonuclease activity of LINEs, carried out by the ORF2p protein, can also mediate reverse transcription and integration of unrelated viral and non-viral RNAs ( Klenerman et al., 1997 Esnault et al., 2000 Buzdin, 2004). Similarly, short interspersed nuclear elements (SINEs), including Alu elements, rely on long interspersed nuclear elements (LINEs) for their reverse transcription and integration ( Coffin et al., 1997 Burns and Boeke, 2012 Feschotte and Gilbert, 2012). For example, mammalian apparent long terminal repeat (LTR)-retrotransposons (MaLRs) rely on endogenous retroviruses (ERVs) for their reverse-transcription and integration. The machinery that mediates reverse transcription and integration of the retroviral and endogenous retroelement genomes can also use alternative RNA templates, creating genomic cDNA copies of the latter. Although diverse classes of RNA viruses create a complementary DNA (cDNA) copy through reverse-transcription of their genomes during their life cycle, integration into the host DNA is a characteristic obligatory step for retroviruses, as well as for endogenous retroelements ( Coffin et al., 1997 Burns and Boeke, 2012 Feschotte and Gilbert, 2012). An extreme form of viral parasitism is the integration of a viral genome DNA copy into the host cell DNA ( Burns and Boeke, 2012 Feschotte and Gilbert, 2012). Viruses hijack the host cell to replicate their RNA or DNA genomes and create progeny virions. Although these findings do not rule out SARS-CoV-2 somatic integration, they nevertheless suggest that human-SARS-CoV-2 chimeric reads found in RNA-seq data may arise during library preparation and do not necessarily signify SARS-CoV-2 reverse transcription, integration in to host DNA and further transcription. Furthermore, chimeric reads between SARS-CoV-2 RNA and RNA transcribed from nuclear DNA were highly enriched for host exonic, rather than intronic or intergenic sequences and often involved the same, highly expressed host genes. Chimeric reads were frequently detected also between SARS-CoV-2 RNA and RNA transcribed from mitochondrial DNA or episomal adenoviral DNA present in transfected cell lines, which was unlikely the result of SARS-CoV-2 integration. Here, we examined the possible origin specifically of human-SARS-CoV-2 chimeric reads in RNA-seq libraries and provide alternative explanations for their origin. One key observation was the presence of chimeric RNA-sequencing (RNA-seq) reads between SARS-CoV-2 RNA and RNA transcribed from human host DNA. High frequency of somatic integration of the RNA virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into the DNA of infected cells was recently suggested, based on a number of observations. The human genome bears evidence of extensive invasion by retroviruses and other retroelements, as well as by diverse RNA and DNA viruses. 2Department of Infectious Disease, St Mary’s Hospital, Imperial College London, London, United Kingdom.1Retroviral Immunology, The Francis Crick Institute, London, United Kingdom.Anastasiya Kazachenka 1 and George Kassiotis 1,2 * The way each development team approaches this kind of app is unique. Based on all the above, could this app reliably hold all your information and processes in one place without requiring you to connect loads of apps together? I was paying attention to speed, how the search function works (and where it searches), as well as other details like the quality of the user interface, onboarding experiences, and available support.Īll-in-one potential. I made sure to evaluate how much control and creativity you can have here. All of these apps run on some form of database, but they vary in the way they give you the power to create views and relationships between data. Can you create to-do lists? Kanban boards? What about leaving comments on documents? Can you mention coworkers?ĭatabase features. How easy is it to create, format, share, and embed content? This blank-slate document vibe is the bread-and-butter of Notion, so I wanted to be sure the alternatives stacked up. But to start, I evaluated all the apps that I tested using the same criteria:ĭocument and note-taking features. Ideally, a great Notion alternative gives you the freedom to create your own productivity system-your network of documents, databases, and references-without forcing an inflexible user experience on you.Ī bit like a sandbox video game, this category of app (which doesn't really have a name yet) gives you the possibility to mix and combine different elements to create your or your team's all-in-one workspace.Įach of the picks on this list has something unique to offer, along with some twists on the original experience you're used to. For more details on our process, read the full rundown of how we select apps to feature on the Zapier blog. We're never paid for placement in our articles from any app or for links to any site-we value the trust readers put in us to offer authentic evaluations of the categories and apps we review. We spend dozens of hours researching and testing apps, using each app as it's intended to be used and evaluating it against the criteria we set for the category. All of our best apps roundups are written by humans who've spent much of their careers using, testing, and writing about software. #lets client know about the servers subnet # will persist these when data connection is brokenĬlient-config-dir "C:\\Program Files\\OpenVPN\\config" # range of address allocated for vpn network Key "C:\\Program Files\\OpenVPN\\easy-rsa\\keys\\ServerVPN.key"ĭh "C:\\Program Files\\OpenVPN\\easy-rsa\\keys\\dh2048.pem" Tls-auth "C:\\Program Files\\OpenVPN\\easy-rsa\\keys\\ta.key" 0Ĭa "C:\\Program Files\\OpenVPN\\easy-rsa\\keys\\ca.crt"Ĭert "C:\\Program Files\\OpenVPN\\easy-rsa\\keys\\ServerVPN.crt" #set to 0 for server and should be 1 on the clients Not entirely sure what went wrong as I followed a tutorial step by step. Socket Buffers: R= S=Īttempting to establish TCP connection with 10.10.10.1:12345 TCP/UDP: Preserving recently used remote address: 10.10.10.1:12345 Incoming Control Channel Authentication: Using 160 bit message hash 'SHA1' for HMAC authentication Outgoing Control Channel Authentication: Using 160 bit message hash 'SHA1' for HMAC authentication Read TCPv4_CLIENT: Unknown error (code=10060) This is the error I received once it went green, the top line was the last line it showed while it was showing green I have configured a server on my host machine and a client on my virtual machine, the server has a green light and so did the client upon initial launch until in the logs it attempts to establish a connection between the server machine 10.10.10.1.Ģ minutes later on the client machine an orange light appears once it tries to establish this TCP connection which it is just stuck at. 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After the Electricians have hung, circuited, and patched the lighting units, the LD will direct the focusing (pointing, shaping and sizing of the light beams) and gelling (coloring) of each unit.Īfter focus has occurred the LD usually sits at a temporary desk (tech table) in the theater (typically on the center line in the middle of the house) where they have a good view of the stage and work with the light board operator, who will either be seated alongside them at a portable control console or talk via headset to the control room. The lighting designer is responsible, in conjunction with the production's independently hired production electrician, who will interface with the theater's master electrician, for directing the theater's electrics crew in the realization of their designs during the technical rehearsals. The LD will discuss the plot with the show's production manager and the theatre's master electrician or technical director to make sure there are no unforeseen problems during load-in. Professional LDs generally use special computer-aided design packages to create accurate and easily readable drafted plots that can be swiftly updated as necessary. The lighting designer uses this paperwork to aid in the visualization of not only ideas but also simple lists to assist the master electrician during load-in, focus, and technical rehearsals. Often, paperwork listing all of this information is also generated by using a program such as Lightwright. Next to each instrument on the plan will be information for any color gel, gobo, or other accessories that need to go with it, and its channel number. The light plot is a scale drawing that communicates the location of lighting fixtures and lighting positions so a team of electricians can independently install the lighting system. Cue sheets are of the most value to stage management. Cue sheets communicate the placement of cues that the LD has created for the show, using artistic terminology rather than technical language, and information on exactly when each cue is called so that the stage manager and the assistants know when and where to call the cue. Examples of typical paperwork include cue sheets, light plots, instrument schedules, shop orders, and focus charts. Various forms of paperwork are essential for the LD to successfully communicate their design to various members of the production team. To help the LD communicate artistic vision, they may employ renderings, storyboards, photographs, reproductions of artwork, or mockups of effects to help communicate how the lighting should look. The LD must take into account the show's mood and the director's vision in creating a lighting design. The LD will also make sure that they have an accurate plan of the theatre's lighting positions and a list of their equipment, as well as an accurate copy of the set design, especially the ground plan and section. The LD will also attend several later rehearsals to observe the way the actors are being directed to use the stage area ('blocking') during different scenes and will receive updates from the stage manager on any changes that occur. The LD will read the script carefully and make notes on changes in place and time between scene-and will have meetings (called design or production meetings) with the director, designers, stage manager, and production manager to discuss ideas for the show and establish budget and scheduling details. At the off-Broadway or off-off-Broadway level, the LD will occasionally be responsible for much of the hands-on technical work (such as hanging instruments, programming the light board, etc.) that would be the work of the lighting crew in a larger theater. Smaller theater companies may have a resident lighting designer responsible for most of the company's productions or rely on a variety of freelance or even volunteer help to light their productions. For a Broadway show, a touring production and most regional and small productions the LD is usually an outside freelance specialist hired early in the production process. The role of the lighting designer varies greatly within professional and amateur theater. |
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